Associate Scientist - Next Generation Sequencing

Associate Scientist – Next Generation Sequencing

MS with 3+ years and BS with 5+ years experience.
A previous experience with building NGS libraries to deeply mine the antibody repertoires sequences is required.
A strong background in molecular biology and able to work with RNA/DNA/Plasmids or cells as the starting material.
Previous experience generating high quality NGS library as established by rigorous PCR condition optimization, QC process and troubleshooting.
Experience performing DNA fragment purification using Blue Pippin and analyze the size using Tape Station 4200. Library quantification using qubit flex and qPCR.
Knowledge of MiSeq/Nextseq sequencing operation and maintenance.

Generation of NGS library (RNAseq, amplicon), from single cell platform (10X, Rhapsody, Parse Bioscience, PIPseq, plate-based) or bulk B cells, perform library QC, library quantification and running/operating Miseq/Nextseq.
Use molecular biology laboratory best practices for RNA isolation, cDNA synthesis, PCR amplification, SPRI bead based clean up and fragment analysis.
Serve as a subject matter expert for library generation and work with a broad enterprise mindset.
Strategize best workflow and design NGS primers to utilize NGS sequencing tools.
Ability to manage several competing high priority project simultaneously.
Document laboratory work using electronic laboratory notebook and follow the previously established protocol.
Lead and evaluate the emerging single cell library generation and DNA sequencing platforms.
Optimize, improve and troubleshoot NGS related workflows to get better sequencing data.
Work independently with efficiency, agility, speed and collaborate to thrive in a high pace, high impact team environment.

Excellent knowledge of details on the platform source of antibodies, repertoires (e.g. transgenic mice/display platforms; immune or naive repertoires) and their nuances is highly desirable.