Research Technician II – Cell Culture

Research Technician II – Cell Culture

Requirements

• Bachelor of Science degree
• Minimum 6 months of technical expertise in cell culture, general lab instrumentation, cell-based assays required.

Responsibilities

• Serves as subject matter expert for the Cell Services Research Technicians
• Assists Research Tech Supervisor in delegation of daily tasks and management of the Inventory Specialist team
• Provide high quality laboratory support to the Cell Services team
• Grow, maintain, and cryopreserve contamination-free mammalian cells (suspension & adherent)
• Preparation of cell pellets for histological processing
• Provide daily, vials of tested, frozen cell lines to scientists
• Isolate and cryopreserve primary PBMCs and further cell subsets from both whole blood and leukopaks
• Isolate a variety of cell types from fresh blood daily in a timely manner
• Isolate serum from whole blood
• Maintain sterile technique while culturing isolated primary cells
• Perform established flow cytometry QC protocols on a routine basis and analyze results
• Generate and analyze data with the highest Data Integrity standards to include identifying and reporting unusual results/outcomes
• Operate and maintain laboratory instrumentation including Mycoplasma onsite testing & clean-up as warranted
• Continue technological improvements to increase productivity and cost-efficiency
• Develop organization skills, presentation skills and attention to detail
• Interact with senior staff members as necessary
• Ensure compliance with all R&D data integrity practices, ethics codes, standards and training, and safety training and practice
• May be requested to provide on-call service (primary and secondary) for specific research units in the lab areas.

Preferred Qualifications

• Previous experience in biotech and/or pharmaceutical drug discovery
• Prior experience isolating primary cells preferred (Stemcell Technologies/Miltenyi)