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Senior Associate – Cell Based Assays
| Company | Sarepta |
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| Location | Andover, MA, USA |
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| Salary | $92000 – $115000 |
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| Type | Full-Time |
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| Degrees | Bachelor’s |
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| Experience Level | Mid Level, Senior |
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Requirements
- B.S. degree in molecular biology, cell biology or virology with 3-5 years relevant experience.
- Understanding of operating and method development using Jess/ Capillary electrophoresis western blotting.
- Working experience in a cGMP environment is required.
- An understanding of molecular techniques including potency-based immuno-assays, ELISA, immunoblotting, qPCR, ddPCR, and cell banking is necessary.
- Hands on experience running protein-based immunoassays and/or qPCR/ddPCR assays.
- Familiarity with primary mammalian cell culture, in particular myoblasts.
- Transductions with AAV vectors and conditions to optimize payload delivery and expression.
- Experience in developing, validating, optimization and deploying cell and molecular biology test methods is highly desired.
- Experience in general laboratory organization, experimental design, and documentation (GDP) with adherence to cGMP is necessary.
- Operate as a part of a team with experience in coordinating and collaborating with peers.
- Attention to details is a must.
- Effective written and verbal communication skills.
Responsibilities
- Optimize cell culture conditions to support execution of cell-based assays
- Support troubleshooting and optimization of cell and molecular assays, including assay development & validations in accordance with cGMP regulations
- Author quality documents including SOPs and test methods
- Prepare, qualify, and release standards for the in vitro potency methods
- Test new cell lines for use in new potency assays
- Maintaining cell banks on cryogenic storage
- Improve robustness and throughput for existing cell assays, integrating automation platforms
- Support the method development, transfer and qualification/validation activities associated with the cell-based potency assays for AAV-based gene therapy products including protein and mRNA-based expression assays
- Create and maintain clear, concise lab records and documentation
- Sample management: Inventory and tracking of incoming and outgoing samples
- Collaborate with team leads and managers to optimize a smooth workflow for collaboration on team activities
- Perform other related duties incidental to the work described
Preferred Qualifications
- Experience in developing, validating, optimization and deploying cell and molecular biology test methods is highly desired.
